Conservation Genetics Lab: Discovering Lemur Diversity
$79.00
Scientists working in the forests of Madagascar may have rediscovered a species of lemur that was thought extinct. Analyze authentic field data and perform gel electrophoresis to make the call.
- Description
- What's Included
- Requirements
- Videos
- Curriculum Downloads
- Safety Data Sheets
Description
Description
Bring your students on an expedition to Madagascar! Analyze morphological data and run electrophoresis gels to determine whether researchers have rediscovered a species of lemur once thought to be extinct.
- Analyze actual field data
- Construct phylogenetic trees from DNA sequence data
- Compare generalist and specialist species facing ecological change
A molecular evolution and ecology lab based directly on our collaborators’ published and unpublished research, this collaboration with the Duke Lemur Center was designed with the goal of bringing molecular techniques to Ecology and Evolution units. As either a quick, single-period gel running lab or a week-long mini-unit, this lab offers flexibility and high-quality curriculum.
- Techniques: Micropipetting, gel electrophoresis, bioinformatics
- Topics: Molecular ecology, consevation genetics, evolution, biotechnology
- Time required: Can be completed in one 45-minute class periods
- Level: General high school through college
What's Included
Reagents for 8 lab groups:
- 8 Simulated lemur DNA samples
- DNA molecular weight marker (Fast DNA Ladder 1)
Storage notes:
- If kept in the freezer, reagents can be stored for 12 months after receipt.
- If kept in the refrigerator, reagents can be stored for 6 months after receipt.
Requirements
Required equipment:
- 2-20 μl variable volume or 10 μl fixed volume micropipettes and tips
- Gel electrophoresis and fluorescence visualization system (e.g. blueGel™)
Gel electrophoresis reagents and plastic tubes are supplied by the user
- Agarose
- Gel electrophoresis buffer (e.g. TBE)
- Fluorescent DNA stain (e.g. SeeGreen™ or GelGreen®)
- Plastic tubes for distributing samples to student groups
The Learning Lab Companion Kit (KT-1510-01) provides sufficient reagents to prepare and run eight gels when using the blueGelTM electrophoresis system, as well as plastic tubes to distribute samples to student groups.
Videos
Curriculum Downloads
- miniPCR Lemur Lab Teacher’s Guide
- miniPCR Lemur Lab Student’s Guide
- miniPCR Lemur Lab Classroom Slides