Publications

Research enabled by miniPCR® technology

A fluorescence viewer for rapid molecular assay readout in space and low-resource terrestrial environments. Misquitta et al., PLoS One (2024). https://doi.org/10.1371/journal.pone.0291158
A PCR Test Using the Mini-PCR Platform and Simplified Product Detection Methods Is Highly Sensitive and Specific to Detect Fasciola hepatica DNA Mixed in Human Stool, Snail Tissue, and Water DNA Specimens. Fernandez-Baca et al., Pathogens (2024). https://doi.org/10.3390/pathogens13060440
Field-based molecular detection of Batrachochytrium dendrobatidis in critically endangered Atelopus toads and aquatic habitats in Ecuador. Riascos-Flores et al., PLOS ONE (2024). https://doi.org/10.1371/journal.pone.0299246
Open Educational Resources for distributed hands-on teaching in molecular biology. Cerda et al., bioRxiv (2024). https://doi.org/10.1101/2024.03.28.587173
Validation of Cell-Free Protein Synthesis Aboard the International Space Station. Kocalar et al., ACS Synth. Biol. (2024). https://doi.org/10.1021/acssynbio.3c00733
MiniPCR as a portable equipment for the molecular diagnosis of american cutaneous leishmaniasis. Castellanos-Gonzalez et al., Acta Trop. (2023). https://doi.org/10.1016/j.actatropica.2023.106926
Detecting Mediterranean White Sharks with Environmental DNA. Jenrette, J.F., et al. Oceanography (2023). https://doi.org/10.5670/oceanog.2023.s1.28
Combination of ultra-rapid DNA purification (PURE) and loop-mediated isothermal amplification (LAMP) for rapid detection of Trypanosoma cruzi DNA in dried blood spots. Longhi et al., PLOS Neg. Trop. Dis. (2023). https://doi.org/10.1371/journal.pntd.0011290
DNA storage in thermoresponsive microcapsules for repeated random multiplexed data access. Nat. Nanotechnol. (2023). https://doi.org/10.1038/s41565-023-01377-4
PNA-Pdx: Versatile Peptide Nucleic Acid-Based Detection of Nucleic Acids and SNPs. Jiang et al., Anal. Chem. (2023). https://doi.org/10.1021/acs.analchem.3c01809
DNA barcoding of sea urchin species in the bird’s head seascape Papua-Indonesia. Dailami et al., IOP Conf. Ser.: Earth Environ. Sci. (2023). DOI 10.1088/1755-1315/1191/1/012002
Before you go: a packing list for portable DNA sequencing of microbiomes and metagenomes. Edwards et al., Microbiology Research (2022). https://doi.org/10.1099/mic.0.001220
Two large structure-forming sponges from opposite North American coasts: a taxonomic review of Arctic–Pacific Mycale (Mycale) loveni and the description of a new Arctic–Atlantic Mycale. Dinn et al., Canadian Journal of Zoology (2023). https://doi.org/10.1139/cjz-2023-0011.
Environmental DNA and visual encounter surveys for amphibian biomonitoring in aquatic environments of the Ecuadorian Amazon. Quilumbaquin W, et al., Aquatic Biology (2023). https://peerj.com/articles/15455/
Laboratory evaluation of the miniature direct-on-blood PCR nucleic acid lateral flow immunoassay (mini-dbPCR-NALFIA), a simplified molecular diagnostic test for Plasmodium. van Dijk NJ, et al., Malar J (2023). doi: 10.1186/s12936-023-04496-4.
Portable nucleic acid tests for rapid detection of monkeypox virus. Bhadra and Ellington, MedRxiv (2022). https://doi.org/10.1101/2022.08.09.22278605
Towards an in situ non-lethal rapid test to accurately detect the presence of the nematode parasite, Anguillicoloides crassus, in European eel, Anguilla anguilla. De Noia et al., Parasitology (2022). https://doi.org/10.1017/s0031182021002146
Rapid in situ identification of biological specimens via DNA amplicon sequencing using miniaturized laboratory equipment. Pomerantz et al., Nature Protocols (2022). https://doi.org/10.1038/s41596-022-00682-x
CREST, a Cas13-Based, Rugged, Equitable, Scalable Testing (CREST) for SARS-CoV-2 Detection in Patient Samples. Aralis et al., Current Protocols, (2022). https://doi.org/10.1002/cpz1.385
A miniPCR-Duplex Lateral Flow Dipstick Platform for Rapid and Visual Diagnosis of Lymphatic Filariae Infection. Phuakrod et al., Diagnostics (Basel), (2021).
A Scalable, Easy-to-Deploy, Protocol for Cas13-Based Detection of SARS-CoV-2 Genetic Material. Rauch et al., JCM, (2021).
Detection of SARS-CoV-2 RNA using RT-LAMP and molecular beacons. Sherrill-Mix et al., Genome Biology, (2021).
A Novel Miniature CRISPR-Cas13 System for SARS-CoV‑2 Diagnostics. ACS Synth. Biol. (2021), 10, 2541−2551.
Morphological stasis masks ecologically divergent coral species on tropical reefs. Bongaerts et al., Curr. Bio. (2021)
Portable PCR field-based detection of sweetpotato viruses. Ssengo et al., African Crop Science Journal (2021). DOI: 10.4314/acsj.v28i3.3
A CRISPR-based assay for the study of eukaryotic DNA repair onboard the International Space Station. Stahl-Rommel et al., PLOS ONE (2021).
Real-Time Culture-Independent Microbial Profiling Onboard the International Space Station Using Nanopore Sequencing. Stahl-Rommel et al., Genes (2021).
Stony Coral Tissue Loss Disease biomarker bacteria identified in corals and overlying 2 waters using a rapid field-based sequencing approach. Becker et al., BioRxiv (2021).
Prevalence of integrons in Enterobacteriaceae obtained from clinical samples. Barns et al., Journal of Microbiology and Antimicrobials (2021).
In search of the RNA world on Mars. Mojarro et al., Geobiology (2021).
Simpler and faster COVID-19 testing: Strategies to streamline SARS-CoV-2 molecular assays. Panpradista et al., EBioMedicine (2021).
Multiplex Isothermal Amplification Coupled with Nanopore Sequencing for Rapid Detection and 2 Mutation Surveillance of SARS-CoV-2. Bi et al., MedRxiv (2021).
Scaling diagnostics in times of COVID-19: Colorimetric Loop-mediated Isothermal Amplification (LAMP) assisted by a 3D-printed incubator for cost-effective and scalable detection of SARS-CoV-2. González-González et al., PLOS ONE (2020).
Developing a Low-Cost, Simple-to-Use Electrochemical Sensor for the Detection of Circulating Tumour DNA in Human Fluids. Attoye et al., Biosensors (2020).
Portable Rabies Virus Sequencing in Canine Rabies Endemic Countries Using the Oxford Nanopore MinION. Gigante et al., Viruses (2020).
Portable and accurate diagnostics for COVID-19: Combined use of the miniPCR thermocycler and a well-plate reader for SARS-Co2 virus detection. Gonzalez-Gonzalez et al., MedRxiv (2020).
Influenza A Virus Field Surveillance at a Swine-Human Interface. Rambo-Martin et al., Clinical Science and Epidemiology (2020).
One‐Step Nucleic Acid Purification and Noise‐Resistant Polymerase Chain Reaction by Electrokinetic Concentration for Ultralow‐Abundance Nucleic Acid Detection. Ouyang and Han, Angewandte Chemie (2020).
Field-Adapted Full Genome Sequencing of Peste-Des-Petits-Ruminants Virus Using Nanopore Sequencing. Torsson et al., Front Vet Sci. (2020).
Metagenomic water quality monitoring with a portable laboratory. Acharya et al., Water Res. (2020).
Next-generation in situ conservation and capacity building in Madagascar using a mobile genetics lab. Blanco et al., BioRxiv (2019).
Nucleic acid detection aboard the International Space Station by colorimetric loop‐mediated isothermal amplification (LAMP) (Rubinfien et al., FASEB BioAdvances, 2019).
Validation of use of the miniPCR thermocycler for Ebola and Zika virus detection. Gonzalez-Gonzalez et al. PLoS One (2019).
Backpack PCR: A point-of-collection diagnostic platform for the rapid detection of Brugia parasites in mosquitoes. Zaky et al., PLOS Neglected Tropical Diseases (2018).
PCR: Thirty-five years and counting. (Science Magazine Technology Feature mentioning miniPCR.)
Successful Amplification of DNA aboard the International Space Station. Boguraev et al., NPJ Microgravity (2017).
Gene expression studies using a miniaturized thermal cycler system on board the International Space Station. Montague et al., PLOS ONE (2018).
Real-time DNA barcoding in a rainforest using nanopore sequencing: opportunities for rapid biodiversity assessments and local capacity building. Pomerantz et al., GigaScience, giy033, (2018). PDF
Rapid identification of infections. Park et al., Science Advances (2016).
A simple, economical protocol for DNA extraction and amplification where there is no lab. Guevara et al., 2017. PDF
Performance of miniPCR^TM mini8, a portable thermal cycler. Kwon et al., 2016.
PCR Heads into the field. Technology feature, Nature Methods 2015, Vol. 12 No. 5